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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
19/04/1995 |
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Data da última atualização: |
19/04/1995 |
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Autoria: |
WANG, H. C.; BAU, J. H. and YEH, D. S. |
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Afiliação: |
Departament of plant Pathology, National Chung Hsing University, Taichung, Taiwan, R.O.C. |
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Título: |
Comparison of the Nuclear Inclusion b Protein and Coat Protein Genes of Five Papaya Ringspot Virus Strains Distinct in Geographic Origin andPathogenicity. |
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Ano de publicação: |
1994 |
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Fonte/Imprenta: |
The American Phytopathological Society vol.84 (10), 1994. |
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ISSN: |
0331-949X |
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Idioma: |
Inglês |
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Conteúdo: |
A complementary DNA library was constructed to the genomic RNA of an isolate of papaya ringspot virus (PRSV), PRSV YK (YK), that represents the most prevalent mosaic strain of Taiwan. A 2.96-kb clone, pYKB9, corresponding to the 3'-region of YK RNA was selected by immunoscreening with the antiserum to the coat protein (CP). The clone had 2,960 nucleotide residuesand represented part of the nuclear inclusion a (NLa) gene, the complete nuclear inclusion b (NLb) gene, the entire CP gene, and the 3'-noncoding region of YK RNA. The nucleotide sequence was compared to those of the 3' regions of the published Hawaii severe strain, PRSV HA (HA), the Hawaii mild strain PRSV HA 5-1 (HA 5-1), the monpapaya-infecting Florida strain, PRSV W(fl) (W(fl), and the monpapaya-infecting Australia strain PRSV W(aust) (W(aust)). The NIb region had 83.1% nucleotide identity and 95.7% amino acid identity compared to thatof HA, with a total of 262 nucleotide differences resulting in only 22 amino acid changes. A similar trend also was found when the NIb region of YK was compared to the partially elucidated NIb regions of HA 5-1and PRSV W(fl), with 84.7 and 85.2% nucleotide identities (147 and 151 nucleotide differences), respectively: both resulting in 97.6% amino acid identity (only eight amino acid changes). Most of the nucleotide differences between the Asian and American strains occurred in wobble bases and, thus, conserved the amino acid residues, suggesting a common selection pressure to keep the same protein conformation. MenosA complementary DNA library was constructed to the genomic RNA of an isolate of papaya ringspot virus (PRSV), PRSV YK (YK), that represents the most prevalent mosaic strain of Taiwan. A 2.96-kb clone, pYKB9, corresponding to the 3'-region of YK RNA was selected by immunoscreening with the antiserum to the coat protein (CP). The clone had 2,960 nucleotide residuesand represented part of the nuclear inclusion a (NLa) gene, the complete nuclear inclusion b (NLb) gene, the entire CP gene, and the 3'-noncoding region of YK RNA. The nucleotide sequence was compared to those of the 3' regions of the published Hawaii severe strain, PRSV HA (HA), the Hawaii mild strain PRSV HA 5-1 (HA 5-1), the monpapaya-infecting Florida strain, PRSV W(fl) (W(fl), and the monpapaya-infecting Australia strain PRSV W(aust) (W(aust)). The NIb region had 83.1% nucleotide identity and 95.7% amino acid identity compared to thatof HA, with a total of 262 nucleotide differences resulting in only 22 amino acid changes. A similar trend also was found when the NIb region of YK was compared to the partially elucidated NIb regions of HA 5-1and PRSV W(fl), with 84.7 and 85.2% nucleotide identities (147 and 151 nucleotide differences), respectively: both resulting in 97.6% amino acid identity (only eight amino acid changes). Most of the nucleotide differences between the Asian and American strains occurred in wobble bases and, thus, conserved the amino acid residues, suggesting a common selection pressure to keep ... Mostrar Tudo |
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Palavras-Chave: |
Amino acid sequence. |
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Thesaurus Nal: |
Potyvirus. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02092naa a2200169 a 4500
001 1633135
005 1995-04-19
008 1994 bl uuuu u00u1 u #d
022 $a0331-949X
100 1 $aWANG, H. C.
245 $aComparison of the Nuclear Inclusion b Protein and Coat Protein Genes of Five Papaya Ringspot Virus Strains Distinct in Geographic Origin andPathogenicity.
260 $c1994
520 $aA complementary DNA library was constructed to the genomic RNA of an isolate of papaya ringspot virus (PRSV), PRSV YK (YK), that represents the most prevalent mosaic strain of Taiwan. A 2.96-kb clone, pYKB9, corresponding to the 3'-region of YK RNA was selected by immunoscreening with the antiserum to the coat protein (CP). The clone had 2,960 nucleotide residuesand represented part of the nuclear inclusion a (NLa) gene, the complete nuclear inclusion b (NLb) gene, the entire CP gene, and the 3'-noncoding region of YK RNA. The nucleotide sequence was compared to those of the 3' regions of the published Hawaii severe strain, PRSV HA (HA), the Hawaii mild strain PRSV HA 5-1 (HA 5-1), the monpapaya-infecting Florida strain, PRSV W(fl) (W(fl), and the monpapaya-infecting Australia strain PRSV W(aust) (W(aust)). The NIb region had 83.1% nucleotide identity and 95.7% amino acid identity compared to thatof HA, with a total of 262 nucleotide differences resulting in only 22 amino acid changes. A similar trend also was found when the NIb region of YK was compared to the partially elucidated NIb regions of HA 5-1and PRSV W(fl), with 84.7 and 85.2% nucleotide identities (147 and 151 nucleotide differences), respectively: both resulting in 97.6% amino acid identity (only eight amino acid changes). Most of the nucleotide differences between the Asian and American strains occurred in wobble bases and, thus, conserved the amino acid residues, suggesting a common selection pressure to keep the same protein conformation.
650 $aPotyvirus
653 $aAmino acid sequence
700 1 $aBAU, J. H. and YEH, D. S.
773 $tThe American Phytopathological Society vol.84 (10), 1994.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
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Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
12/02/2015 |
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Data da última atualização: |
31/07/2017 |
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Tipo da produção científica: |
Folder/Folheto/Cartilha |
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Autoria: |
ROCHA, H. S.; ARAUJO, J. C. de; SILVA, A. C. M. da; OLIVEIRA, S. A. S. de; BORGES, A. L.; FERREIRA FILHO, J. R.; MEISSNER FILHO, P. E.; SILVEIRA, H. F. da; RINGENBERG, R.; CARDOSO, C. E. L. |
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Afiliação: |
HERMINIO SOUZA ROCHA, CNPMF; JACQUELINE CAMOLESE DE ARAUJO, CNPMF; AUGUSTO CESAR MOURA DA SILVA, CNPMF; SAULO ALVES SANTOS DE OLIVEIRA, CNPMF; ANA LUCIA BORGES, CNPMF; JOSÉ RAIMUNDO FERREIRA FILHO, EBDA; PAULO ERNESTO MEISSNER FILHO, CNPMF; HELTON FLECK DA SILVEIRA, CNPMF; RUDINEY RINGENBERG, CNPMF; CARLOS ESTEVAO LEITE CARDOSO, CNPMF. |
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Título: |
Recomendações técnicas para a produção de manivas-semente de mandioca a partir de mudas micropropagadas. O papel do "maniveiro" - Projeto RENIVA. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Cruz das Almas, BA: Embrapa Mandioca e Fruticultura, 2014. |
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Idioma: |
Português |
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Conteúdo: |
A muda ou semente de qualidade constitui-se no principal insumo agrícola para a sustentação de uma produção, quando se visa alcançar níveis ótimos de produtividade e longevidade da cultura. O projeto RENIVA (Rede de multiplicação e transferência de manivas-semente de mandioca com qualidade genética e fitossanitária) objetiva constituir uma rede de multiplicação e distribuição de manivas-semente de mandioca com qualidade genética e fitossanitária, tanto para pequenos agricultores familiares quanto para os grandes agricultores das principais regiões produtoras de mandioca em todo o território nacional. |
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Thesagro: |
Mandioca; Micropropagação; Reprodução vegetal. |
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Thesaurus NAL: |
Cassava. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/127471/1/Cartilha-Reniva-28.04.2015-1.pdf
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Marc: |
LEADER 01469nam a2200265 a 4500
001 2008597
005 2017-07-31
008 2014 bl uuuu u0uu1 u #d
100 1 $aROCHA, H. S.
245 $aRecomendações técnicas para a produção de manivas-semente de mandioca a partir de mudas micropropagadas. O papel do "maniveiro" - Projeto RENIVA.
260 $aCruz das Almas, BA: Embrapa Mandioca e Fruticultura$c2014
520 $aA muda ou semente de qualidade constitui-se no principal insumo agrícola para a sustentação de uma produção, quando se visa alcançar níveis ótimos de produtividade e longevidade da cultura. O projeto RENIVA (Rede de multiplicação e transferência de manivas-semente de mandioca com qualidade genética e fitossanitária) objetiva constituir uma rede de multiplicação e distribuição de manivas-semente de mandioca com qualidade genética e fitossanitária, tanto para pequenos agricultores familiares quanto para os grandes agricultores das principais regiões produtoras de mandioca em todo o território nacional.
650 $aCassava
650 $aMandioca
650 $aMicropropagação
650 $aReprodução vegetal
700 1 $aARAUJO, J. C. de
700 1 $aSILVA, A. C. M. da
700 1 $aOLIVEIRA, S. A. S. de
700 1 $aBORGES, A. L.
700 1 $aFERREIRA FILHO, J. R.
700 1 $aMEISSNER FILHO, P. E.
700 1 $aSILVEIRA, H. F. da
700 1 $aRINGENBERG, R.
700 1 $aCARDOSO, C. E. L.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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